Journal: Archives of oral biology

Article Title: Genomic integration and expression of the Aggregatibacter actinomycetemcomitans catalase gene in Aggregatibacter aphrophilus

doi: 10.1016/j.archoralbio.2017.12.002

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: As needed, spectinomycin (Sigma-Aldrich, St. Louis, MO, USA), rifampicin (Sigma-Aldrich) or ampicillin (Sigma-Aldrich) were added to the media at concentrations of either 50 μg/ml or 100 μg/ml. table ft1 table-wrap mode="anchored" t5 caption a7 Strains and plasmids Property/relevant genotype Source/reference STRAINS A. actinomycetemcomitans ATCC29523 Laboratory strains ATCC (Manassas, VA, USA) * D7S-1 Wildtype A. actinomycetemcomitans, serotype a Oral isolation (Chen et al., 2010) D7S-Rif r Rifampicin resistant This study D7S-dkat-Sp r D7S-1 Δ( katA )::Sp r This study D7S-dkat D7S-1 Δ( katA ) This study D7S-dkatC D7S-1 Δ( katA ):: katA This study A. aphrophilus NJ8700 Wildtype A. aphrophilus Sequenced strain ( Di Bonaventura et al., 2009 ) NJ8700-Rif r Rifampicin resistant This study NJ-kat + -Sp r NJ8700:: katA- Sp r This study NJ-kat + NJ8700:: katA This study ATCC19415, ATCC29241, ATCC29242, ATCC33389, ATCC13252 Laboratory strains ATCC RMA1185, RMA4465, RMA7085, RMA9045, {"type":"entrez-protein","attrs":{"text":"RMA12201","term_id":"1496124402","term_text":"RMA12201"}} RMA12201 , {"type":"entrez-protein","attrs":{"text":"RMA12676","term_id":"1496124886","term_text":"RMA12676"}} RMA12676 , {"type":"entrez-protein","attrs":{"text":"RMA12929","term_id":"1496125144","term_text":"RMA12929"}} RMA12929 Clinical isolates R. M. Alden Research Laboratory (Culver City, CA, USA) S. gordonii Challis ATCC35105 Laboratory strains Gift from Dr. Elaine Haase (SUNY Buffalo, Buffalo, NY, USA) E. Coli DH5α Host for cloning/expression vector pBluescript II KS + Promega BioSciences (San Luis Obispo, CA, USA) PLASMIDS pBluescript II KS + Cloning vector, Amp r Stratagene (San Diego, CA, USA) pLox-Sp r Amp r and Sp r in pBluescript II KS + , containing two loxP sites (spacer sequence: ATGGATGC) flanking the Sp r gene Laboratory collection( Cheng et al., 2014 ) PCAY-1 gatA (NJ8700_00310) from NJ8700 cloned into pLox-Sp r , Amp r and Sp r This study PCAY-2 gdhA (D7S_00738) and katA (D7S_00739) from D7S-1 cloned into pCAY-1, Amp r and Sp r This study Open in a separate window * ATCC, American Type Culture Collection Bacterial strains and plasmids The spontaneous rifampicin-resistant mutants A. actinomycetemcomitans D7S-Rif r and A. aphrophilus NJ8700-Rif r were generated by plating the A. actinomycetemcomitans D7S-1 and A. aphrophilus NJ8700 wildtype strains on mTSB and chocolate agar, respectively, supplemented with 50 μg/ml rifampicin.

Techniques: Isolation, Cloning, Plasmid Preparation, Sequencing, Clone Assay

Journal: Clinical and Vaccine Immunology

Article Title: Genetic and Antigenic Diversity of the Surface Protective Antigen Proteins of Erysipelothrix rhusiopathiae

doi: 10.1128/cvi.00099-07

Figure Lengend Snippet: FIG. 4. Results of immunoblot analysis of E. rhusiopathiae whole- cell proteins from different serovars probed with polyclonal anti-rSpaA (A), anti-rSpaB (B), and anti-rSpaC (C) antibodies. Lanes: 1, MEW 22 (type N); 2, 545 (serovar 17); 3, Tanzania (serovar 16); 4, Pe’cs 3597 (serovar 15); 5, Kaparek (serovar 9); 6, Pe’cs 9 (serovar 12); 7, Goda (serovar 8); 8, Fujisawa (serovar 1a); 9, 422/1E1 (serovar 1b); 10, ATCC 19414T (serovar 2); 11, Pe’cs 67 (serovar 5); 12, Doggerscharbe (serovar 4); 13, Dolphin E-1 (serovar 6); 14, IV12/8 (serovar 11); 15, 2017 (serovar 19); 16, Ba˜no 36 (serovar 21); and 17, 715 (serovar 18).

Article Snippet: Similar results were observed when mice were challenged with the serovar 2 strain ATCC 19414T, which expresses FIG. 6.

Techniques: Western Blot

Journal: Clinical and Vaccine Immunology

Article Title: Genetic and Antigenic Diversity of the Surface Protective Antigen Proteins of Erysipelothrix rhusiopathiae

doi: 10.1128/cvi.00099-07

Figure Lengend Snippet: FIG. 7. Cumulative mortality of nonimmunized mice and mice immunized with rSpa proteins after challenge with homologous and heterol- ogous serovar strains. (A) Challenge with serovar 1a strain Fujisawa producing SpaA; (B) challenge with serovar 2 strain ATCC 19414T producing SpaA; (C) challenge with serovar 6 strain Dolphin E-1 producing SpaB; (D) challenge with serovar 18 strain 715 producing SpaC.

Article Snippet: Similar results were observed when mice were challenged with the serovar 2 strain ATCC 19414T, which expresses FIG. 6.

Techniques:

Journal: Poultry Science

Article Title: In vitro evaluation of the inhibitory potential of specific egg yolk antibodies induced by different antigens of Salmonella Typhimurium

doi: 10.1016/j.psj.2025.105934

Figure Lengend Snippet: The cross-reactivity of anti S . Typhimurium IgY with other Enterobacteriaceae family including S. Infantis and S. Enteritidis. E. coli O2 and O78K80, Campylobacter jejuni and fetus , and Staphylococcus aureus. UIA (ultrasonicated-inactivated antigen), FIA (formalin-inactivated antigen). Error bars indicate the mean ± standard deviation ( n = 6 samples/treatment groups).

Article Snippet: ELISA plates were coated with inactivated Escherichia coli O2 (ATCC: 700728), Escherichia coli O78k80 (ATCC: 19413), Salmonella Enteritidis (ATCC: 13076), Salmonella Infantis (ATCC: 51741), Campylobacter jejuni (ATCC: 29428) and fetus (ATCC: 27374), and Staphylococcus aureus as antigens.

Techniques: Standard Deviation

Journal: Journal of dairy science

Article Title: Molecular identification and further characterization of Arcanobacterium pyogenes isolated from bovine mastitis and from various other origins.

doi: 10.3168/jds.2010-3678

Figure Lengend Snippet: Figure 2. Typical amplicons using the Arcanobacterium pyogenes sodA gene-specific oligonucleotide primer pair. A. pyogenes DSM 20630T (1) and 2 A. pyogenes isolates of the present study (2, 3) with a size of approximately 200 bp. Negative reaction of Arcanobacterium abortisuis DSM 19515T (4). M = GeneRuler 100-bp DNA ladder (Fermentas, St. Leon-Rot, Germany).

Article Snippet: However, in contrast to Silva et al. (2008) and Santos et al. (2010), in the present study, cbpA could also be found for reference strain A. pyogenes DSM 20630T (ATCC 19411), originally isolated from a pig.

Techniques: